pcDNA3.1-hSTING (S366A)
(Plasmid
#214155)
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PurposeTransient expression of STING
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214155 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTING (S366A)
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SpeciesH. sapiens (human)
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MutationS366A
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Entrez GeneSTING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer BGH_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-hSTING (S366A) was a gift from Nan Yan (Addgene plasmid # 214155 ; http://n2t.net/addgene:214155 ; RRID:Addgene_214155) -
For your References section:
Interferon-Independent Activities of Mammalian STING Mediate Antiviral Response and Tumor Immune Evasion. Wu J, Dobbs N, Yang K, Yan N. Immunity. 2020 Jul 14;53(1):115-126.e5. doi: 10.1016/j.immuni.2020.06.009. Epub 2020 Jul 7. 10.1016/j.immuni.2020.06.009 PubMed 32640258