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Addgene

pMRX-hSTING (N154S)-EGFP
(Plasmid #214150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STING
  • Species
    H. sapiens (human)
  • Entrez Gene
    STING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGGACCATCCTCTAGACTG
  • 3′ sequencing primer CGATACTTGGCGTAATCAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-hSTING (N154S)-EGFP was a gift from Nan Yan (Addgene plasmid # 214150 ; http://n2t.net/addgene:214150 ; RRID:Addgene_214150)

  • For your References section:

    Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C. Chu TT, Tu X, Yang K, Wu J, Repa JJ, Yan N. Nature. 2021 Aug;596(7873):570-575. doi: 10.1038/s41586-021-03762-2. Epub 2021 Jul 21. 10.1038/s41586-021-03762-2 PubMed 34290407
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