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delCMV-mCherry-2x-Rhotekin-GBD
(Plasmid #214142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    delCMV-mCherry
  • Total vector size (bp) 4776
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xRhotekin-GBD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    246
  • Mutation
    Only the GTPase binding domain (GBD) is included
  • Entrez Gene
    Rtkn
  • Promoter delCMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the delCMV promotor was derived from Watanabe and Mitchison, 2002, Science, 295:1083

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delCMV-mCherry-2x-Rhotekin-GBD was a gift from Leif Dehmelt (Addgene plasmid # 214142 ; http://n2t.net/addgene:214142 ; RRID:Addgene_214142)

  • For your References section:

    Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. Nanda S, Calderon A, Sachan A, Duong TT, Koch J, Xin X, Solouk-Stahlberg D, Wu YW, Nalbant P, Dehmelt L. Nat Commun. 2023 Dec 15;14(1):8356. doi: 10.1038/s41467-023-43875-y. 10.1038/s41467-023-43875-y PubMed 38102112
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