delCMV-mCherry-3x-p67phox-GBD
(Plasmid #214141)

• Purpose: Rac activity sensor
• Depositing Lab: Leif Dehmelt
• Publication: Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi: 10.1038/s41467-023-43875-y.

Backbone

• Vector backbone: delCMV-mCherry
• Total vector size (bp): 6120
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xp67phox-GBD
• Alt name: NCF2
• Species: H. sapiens (human)
• Insert Size (bp): 609
• Mutation: Only the GTPase binding domain (GBD) is included
• Entrez Gene: NCF2 (a.k.a. NCF-2, NOXA2, P67-PHOX, P67PHOX)
• Promoter: delCMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • delCMV-mCherry-3x-p67phox-GBD.gb
• A portion of this plasmid was derived from a plasmid made by: Prof. Reza Ahmadian, Heinrich Heine University, Düsseldorf, Germany

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

the delCMV promotor was derived from Watanabe and Mitchison, 2002, Science, 295:1083