delCMV-mCherry-3x-p67phox-GBD
(Plasmid
#214141)
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PurposeRac activity sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonedelCMV-mCherry
- Total vector size (bp) 6120
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xp67phox-GBD
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Alt nameNCF2
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SpeciesH. sapiens (human)
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Insert Size (bp)609
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MutationOnly the GTPase binding domain (GBD) is included
- Promoter delCMV
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer whole plasmid sequencing (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byProf. Reza Ahmadian, Heinrich Heine University, Düsseldorf, Germany
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
the delCMV promotor was derived from Watanabe and Mitchison, 2002, Science, 295:1083
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
delCMV-mCherry-3x-p67phox-GBD was a gift from Leif Dehmelt (Addgene plasmid # 214141 ; http://n2t.net/addgene:214141 ; RRID:Addgene_214141) -
For your References section:
Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. Nanda S, Calderon A, Sachan A, Duong TT, Koch J, Xin X, Solouk-Stahlberg D, Wu YW, Nalbant P, Dehmelt L. Nat Commun. 2023 Dec 15;14(1):8356. doi: 10.1038/s41467-023-43875-y. 10.1038/s41467-023-43875-y PubMed 38102112