pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII
(Plasmid #214139)

• Purpose: Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2
• Depositing Lab: Brenda Schulman
• Publication: Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.

Backbone

• Vector backbone: pACEBac1
• Total vector size (bp): 11848
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: PSMB1
• Species: H. sapiens (human)
• Entrez Gene: PSMB1 (a.k.a. HC5, NEDMHAL, PMSB1, PSC5)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: PSMB2
• Species: H. sapiens (human)
• Entrez Gene: PSMB2 (a.k.a. HC7-I)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: PSMB3
• Species: H. sapiens (human)
• Entrez Gene: PSMB3 (a.k.a. HC10-II)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: PSMB4
• Species: H. sapiens (human)
• Entrez Gene: PSMB4 (a.k.a. HN3, HsN3, PRAAS3, PROS-26, PROS26)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 5

• Gene/Insert name: PSMB5
• Species: H. sapiens (human)
• Entrez Gene: PSMB5 (a.k.a. LMPX, MB1)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 6

• Gene/Insert name: PSMB6
• Species: H. sapiens (human)
• Entrez Gene: PSMB6 (a.k.a. DELTA, LMPY)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 6

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 7

• Gene/Insert name: PSMB7
• Species: H. sapiens (human)
• Entrez Gene: PSMB7
• Promoter: polyhedrin
• Tag / Fusion Protein:
  • 2xSTII (C terminal on insert)

Cloning Information for Gene/Insert 7

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there is a deletion in Gentamycin that causes slightly reduced growth efficiency on (+Amp/Kan/Gen/Tet) compared to cells with a fully WT Gen resistance gene.