pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
(Plasmid #214137)

• Purpose: Insect cell expression of the human 20SCP proteasome assembly chaperones
• Depositing Lab: Brenda Schulman
• Publication: Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.

Backbone

• Vector backbone: pACEBac1
• Total vector size (bp): 8084
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: POMP
• Species: H. sapiens (human)
• Entrez Gene: POMP (a.k.a. C13orf12, HSPC014, PNAS-110, PRAAS2, UMP1)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: PSMG1
• Species: H. sapiens (human)
• Entrez Gene: PSMG1 (a.k.a. C21LRP, DSCR2, LRPC21, PAC-1, PAC1)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: PSMG2
• Species: H. sapiens (human)
• Entrez Gene: PSMG2 (a.k.a. CLAST3, HCCA3, HsT1707, MDS003, PAC2, PRAAS4, TNFSF5IP1)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: PSMG3
• Species: H. sapiens (human)
• Entrez Gene: PSMG3 (a.k.a. C7orf48, PAC3)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 5

• Gene/Insert name: PSMG4
• Species: H. sapiens (human)
• Entrez Gene: PSMG4 (a.k.a. C6orf86, PAC4, bA506K6.2)
• Promoter: polyhedrin

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there is a deletion in Gentamycin that causes slightly reduced growth efficiency on (+Amp/Kan/Gen/Tet) compared to cells with a fully WT Gen resistance gene.