pLenti-uORF1-MET
(Plasmid
#214104)
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PurposeMET 5'UTR upstream of mGFP (uORF1 mutated)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMET 5'UTR
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SpeciesH. sapiens (human)
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Insert Size (bp)396
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MutationuORF1 mutation
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GenBank IDNM_000245.4
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Tag
/ Fusion Protein
- GFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-uORF1-MET was a gift from Paolo Comoglio (Addgene plasmid # 214104 ; http://n2t.net/addgene:214104 ; RRID:Addgene_214104) -
For your References section:
The integrated stress response drives MET oncogene overexpression in cancers. Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM. EMBO J. 7 Jan 2025. doi: 10.1038/s44318-024-00338-4. 10.1038/s44318-024-00338-4