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Addgene

pLenti_5'UTR_MET_GFP
(Plasmid #214103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MET 5'UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    396
  • GenBank ID
    NM_000245
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCMV5F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_5'UTR_MET_GFP was a gift from Paolo Comoglio (Addgene plasmid # 214103 ; http://n2t.net/addgene:214103 ; RRID:Addgene_214103)

  • For your References section:

    The integrated stress response drives MET oncogene overexpression in cancers. Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM. EMBO J. 7 Jan 2025. doi: 10.1038/s44318-024-00338-4. 10.1038/s44318-024-00338-4
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