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lenti-EGFR-L858R-T790M-dual-nick sgRNA
(Plasmid #214101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti-sgRNA blast (Plasmid #104993)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
  • gRNA/shRNA sequence
    TTACTTTGCCTCCTTCTGCA; CCTCCAGGAAGCCTACGTGA
  • Species
    Synthetic
  • Promoter hU6

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer attacagggacagcagagatccag
  • 3′ sequencing primer actgtgggcgatgtgcgctctgccc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-EGFR-L858R-T790M-dual-nick sgRNA was a gift from Ting Zhou (Addgene plasmid # 214101 ; http://n2t.net/addgene:214101 ; RRID:Addgene_214101)

  • For your References section:

    A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cells. Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, Zhou T. bioRxiv [Preprint]. 2024 Jan 19:2024.01.18.576233. doi: 10.1101/2024.01.18.576233. 10.1101/2024.01.18.576233 PubMed 38293122
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