lenti-tmpknot-epegRNA-puro backbone
(Plasmid
#214089)
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Purpose(Empty Backbone) Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
- Backbone size (bp) 9200
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Vector typeMammalian Expression, Lentiviral
- Promoter hU6, EF1a
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsPick pink/red colonies.
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lenti-tmpknot-epegRNA-puro backbone was a gift from Ting Zhou (Addgene plasmid # 214089 ; http://n2t.net/addgene:214089 ; RRID:Addgene_214089) -
For your References section:
A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cells. Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, Zhou T. bioRxiv [Preprint]. 2024 Jan 19:2024.01.18.576233. doi: 10.1101/2024.01.18.576233. 10.1101/2024.01.18.576233 PubMed 38293122
Map uploaded by the depositor.
