ART
(Plasmid
#214063)
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PurposeFluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRD_RIPE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTurboRFP
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MutationLinker sequence present between RFP and GFP
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ART was a gift from Sujatha Jagannathan (Addgene plasmid # 214063 ; http://n2t.net/addgene:214063 ; RRID:Addgene_214063) -
For your References section:
A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay. Kolakada D, Campbell AE, Galvis LB, Li Z, Lore M, Jagannathan S. Nucleic Acids Res. 2024 Feb 20:gkae121. doi: 10.1093/nar/gkae121. 10.1093/nar/gkae121 PubMed 38375914
