AAVS1-iPE-Plus
(Plasmid #214062)

• Purpose: inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus
• Depositing Lab: Ting Zhou
• Publication: Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.18.576233. doi: 10.1101/2024.01.18.576233.

Backbone

• Vector backbone: AAVS1 homologous recombination donor plasmid
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE-Plus
• Alt name: PEmax-P2A-hP53DD-IRES-hMLH1dn
• Species: H. sapiens (human), Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
• Insert Size (bp): 9596
• GenBank ID:
• Promoter: TRE-tight

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCTCGTTTAGTGAACCGTCAGATC
• 3′ sequencing primer: AATTCTGCAGATAAACGCTG

Resource Information

• Supplemental Documents:
  • AAVS1-iPE-Plus.gbk
  • AAVS1-iPE-Plus.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry