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Addgene

pEMJ_042
(Plasmid #214061)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214061 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRSET
  • Backbone size w/o insert (bp) 2955
  • Total vector size (bp) 4212
  • Modifications to backbone
    Removal of some sequence 3' of insert.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LanTERN
  • Species
    Synthetic
  • Insert Size (bp)
    1257
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • T7 Tag (N terminal on backbone)
    • Xpress(tm) Tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMJ_042 was a gift from Pamela Silver (Addgene plasmid # 214061 ; http://n2t.net/addgene:214061 ; RRID:Addgene_214061)
  • For your References section:

    LanTERN: A Fluorescent Sensor That Specifically Responds to Lanthanides. Jones EM, Su Y, Sander C, Justman QA, Springer M, Silver PA. ACS Synth Biol. 2024 Feb 20. doi: 10.1021/acssynbio.3c00600. 10.1021/acssynbio.3c00600 PubMed 38377571