pOPINE-3C-mCherry
(Plasmid
#214060)
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PurposeExpression of fluorescent fusion proteins
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOPINE
- Backbone size w/o insert (bp) 5534
- Total vector size (bp) 6262
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
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Insert Size (bp)728
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Tag
/ Fusion Protein
- Hexahistidine (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
- 3′ sequencing primer TCG ATC TCA GTG GTA TTT GTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINE-3C-mCherry was a gift from Ray Owens (Addgene plasmid # 214060 ; http://n2t.net/addgene:214060 ; RRID:Addgene_214060)
Map uploaded by the depositor.
