Skip to main content
Addgene

pNon-target
(Plasmid #214053)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214053 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC
  • Vector type
    Bacterial Expression ; non-target RNA plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Non-target sequence
  • Species
    Synthetic
  • Promoter trc promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.23.546230 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNon-target was a gift from Raymond Staals (Addgene plasmid # 214053 ; http://n2t.net/addgene:214053 ; RRID:Addgene_214053)

  • For your References section:

    Type III-B CRISPR-Cas cascade of proteolytic cleavages. Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, Kostlbacher S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ. Science. 2024 Feb 2;383(6682):512-519. doi: 10.1126/science.adk0378. Epub 2024 Feb 1. 10.1126/science.adk0378 PubMed 38301007
Related items:
Commonly requested with: