pGL3b(1454/3172)P2P-mut
(Plasmid #21405)

• Depositing Lab: Eric Metzen
• Publication: Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGLN1
• Species: H. sapiens (human)
• Insert Size (bp): 1720
• Mutation: hypoxia response element mutated
• Entrez Gene: EGLN1 (a.k.a. C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

oligo used to generate the mutant construct is - 5'-CCGTGGGGCCGTGGTGTAataataGAGCGCGCAGAGCGAGTG