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pJS-BCD-Strep-SAVED-CHAT-cbm
(Plasmid #214032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAVED-CHAT
  • Species
    Haliangium ochraceum DSM 14367
  • Mutation
    K121E, Q122A, N274A, R276E, Y285A, F286A
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • Strep-tag II (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.23.546230 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJS-BCD-Strep-SAVED-CHAT-cbm was a gift from Raymond Staals (Addgene plasmid # 214032 ; http://n2t.net/addgene:214032 ; RRID:Addgene_214032)

  • For your References section:

    Type III-B CRISPR-Cas cascade of proteolytic cleavages. Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, Kostlbacher S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ. Science. 2024 Feb 2;383(6682):512-519. doi: 10.1126/science.adk0378. Epub 2024 Feb 1. 10.1126/science.adk0378 PubMed 38301007
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