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Addgene

AAVS1-iPE2-Hygro
(Plasmid #214019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1 homologous recombination donor plasmid
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2
  • Species
    Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    6354
  • Promoter TRE-tight

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
  • 3′ sequencing primer AATTCTGCAGATAAACGCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-iPE2-Hygro was a gift from Ting Zhou (Addgene plasmid # 214019 ; http://n2t.net/addgene:214019 ; RRID:Addgene_214019)

  • For your References section:

    A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cells. Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, Zhou T. bioRxiv [Preprint]. 2024 Jan 19:2024.01.18.576233. doi: 10.1101/2024.01.18.576233. 10.1101/2024.01.18.576233 PubMed 38293122
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