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pAAVS1-Neo-TNNT2-Hygro
(Plasmid #214015)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAVS1-Nst-CAG-DEST (Addgene Plasmid #80489)
  • Backbone manufacturer
    Knut Woltjen
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    In mammalian cells, use Neomycin for selection of integrated constructs and use Hygromycin for selection of cells with the TNNT2 promoter active
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNNT2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TNNT2 (a.k.a. CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT)

Cloning Information

  • Cloning method TOPO Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1-Neo-TNNT2-Hygro was a gift from Paul Burridge (Addgene plasmid # 214015 ; http://n2t.net/addgene:214015 ; RRID:Addgene_214015)

  • For your References section:

    Independent compartmentalization of functional, metabolic, and transcriptional maturation of hiPSC-derived cardiomyocytes. Fetterman KA, Blancard M, Lyra-Leite DM, Vanoye CG, Fonoudi H, Jouni M, DeKeyser JL, Lenny B, Sapkota Y, George AL Jr, Burridge PW. Cell Rep. 2024 May 28;43(5):114160. doi: 10.1016/j.celrep.2024.114160. Epub 2024 Apr 27. 10.1016/j.celrep.2024.114160 PubMed 38678564
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