pEM7065
(Plasmid
#214003)
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PurposePrpsM driven expression of ssrA-tagged mCerulean (LVA)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKH70-PrpsM
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCerulean-LVA
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SpeciesSynthetic
- Promoter PrpsM
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEM7065 was a gift from Marc Erhardt (Addgene plasmid # 214003 ; http://n2t.net/addgene:214003 ; RRID:Addgene_214003) -
For your References section:
Fluorescent tools for the standardized work in Gram-negative bacteria. Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF. J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9. 10.1186/s13036-024-00420-9 PubMed 38589953