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Addgene

Luciferase
(Plasmid #213979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcW57.1
  • Backbone size w/o insert (bp) 7702
  • Total vector size (bp) 9352
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral
  • Selectable markers
    Puromycin, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    Firefly
  • Insert Size (bp)
    1650
  • GenBank ID
    NW_022170249.1
  • Promoter Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter
  • Tag / Fusion Protein
    • 6xHis affinity tag

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer tcgaggtaggcgtgtacggtgg
  • 3′ sequencing primer aatagggttggcaccagcagcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Luciferase was a gift from Coleman Lindsley (Addgene plasmid # 213979 ; http://n2t.net/addgene:213979 ; RRID:Addgene_213979)

  • For your References section:

    The clinical and functional effects of TERT variants in myelodysplastic syndrome. Reilly CR, Myllymaki M, Redd R, Padmanaban S, Karunakaran D, Tesmer V, Tsai FD, Gibson CJ, Rana HQ, Zhong L, Saber W, Spellman SR, Hu ZH, Orr EH, Chen MM, De Vivo I, DeAngelo DJ, Cutler C, Antin JH, Neuberg D, Garber JE, Nandakumar J, Agarwal S, Lindsley RC. Blood. 2021 Sep 9;138(10):898-911. doi: 10.1182/blood.2021011075. 10.1182/blood.2021011075 PubMed 34019641
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