pAAV-pCALM1-EYFP-WPRE-bGHpA
(Plasmid
#213971)
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PurposeAAV expression of EYFP from pCALM1 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV-hSyn1-EYFP
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCALM1
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-pCALM1-EYFP-WPRE-bGHpA was a gift from Zhonghua Lu (Addgene plasmid # 213971 ; http://n2t.net/addgene:213971 ; RRID:Addgene_213971) -
For your References section:
An ultra-compact promoter drives widespread neuronal expression in mouse and monkey brains. Wang J, Lin J, Chen Y, Liu J, Zheng Q, Deng M, Wang R, Zhang Y, Feng S, Xu Z, Ye W, Hu Y, Duan J, Lin Y, Dai J, Chen Y, Li Y, Luo T, Chen Q, Lu Z. Cell Rep. 2023 Nov 28;42(11):113348. doi: 10.1016/j.celrep.2023.113348. Epub 2023 Oct 31. 10.1016/j.celrep.2023.113348 PubMed 37910509