pcg 2.0-BFP-circ-arRNA backbone
(Plasmid
#213965)
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Purpose(Empty Backbone) pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcg2.0
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Vector typeMammalian Expression
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Selectable markersBFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcg 2.0-BFP-circ-arRNA backbone was a gift from Wensheng Wei (Addgene plasmid # 213965 ; http://n2t.net/addgene:213965 ; RRID:Addgene_213965) -
For your References section:
Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo. Yi Z, Qu L, Tang H, Liu Z, Liu Y, Tian F, Wang C, Zhang X, Feng Z, Yu Y, Yuan P, Yi Z, Zhao Y, Wei W. Nat Biotechnol. 2022 Jun;40(6):946-955. doi: 10.1038/s41587-021-01180-3. Epub 2022 Feb 10. 10.1038/s41587-021-01180-3 PubMed 35145313
