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pCMV-ARMS1-ProLink2-MRGPRX3
(Plasmid #213962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-ARMS1-ProLinkTM 2
  • Backbone manufacturer
    Eurofins DiscoverX, Fremont, CA, USA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418), Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MRGPRX3
  • Entrez Gene
    MRGPRX3 (a.k.a. GPCR, MRGX3, SNSR1, SNSR2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer GCATAATGCTAGCACCATGGATTCAACCATCCCAGTCTTG
  • 3′ sequencing primer TTCGAAGCTTGACTGCTCCAATCTGCTTCCCGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Christa Müller research group

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-ARMS1-ProLink2-MRGPRX3 was a gift from Christa Müller (Addgene plasmid # 213962 ; http://n2t.net/addgene:213962 ; RRID:Addgene_213962)

  • For your References section:

    Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2. Al Hamwi G, Namasivayam V, Buschbell B, Gedschold R, Golz S, Muller CE. Commun Biol. 2024 Jan 6;7(1):52. doi: 10.1038/s42003-023-05739-5. 10.1038/s42003-023-05739-5 PubMed 38184723
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