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Addgene

pDONR221_zCas9
(Plasmid #213913)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213913 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    zCas9
  • Alt name
    3xFLAG-NLS-zCas9-NLS
  • Species
    S. pyogenes
  • Mutation
    Zea mays codon-optimized Cas9

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221_zCas9 was a gift from Sébastien Mongrand (Addgene plasmid # 213913 ; http://n2t.net/addgene:213913 ; RRID:Addgene_213913)

  • For your References section:

    Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis. Ziqiang P. Li, Jennifer Huard, Emmanuelle M. Bayer, Valérie Wattelet-Boyer. Bio Protocol, Vol 14, Iss 13, Jul 5, 2024 10.21769/BioProtoc.5029
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