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Addgene

pHelper_TetR/pTet-Tnpase (carbR)
(Plasmid #213911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHdCas9
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TetR/ptet ATc-inducible himar1C9 transposase
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from pHdCas9 (addgene #137080)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains an ATc-inducible himar1C9 transposase. The backbone encodes for ampicillin/carbenicillin resistance on a medium copy p15A backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelper_TetR/pTet-Tnpase (carbR) was a gift from James Collins (Addgene plasmid # 213911 ; http://n2t.net/addgene:213911 ; RRID:Addgene_213911)

  • For your References section:

    A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks. English MA, Alcantar MA, Collins JJ. Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 10.15252/msb.202211398. Epub 2023 Mar 27. 10.15252/msb.202211398 PubMed 36970845
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