Skip to main content
Addgene

pHelper_AraC/pBad-Tnpase (camR)
(Plasmid #213909)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSAM_AraC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AraC/pBad ATc-inducible himar1C9 transposase
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from pSAM_AraC (addgene #91569)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains an arabinose-inducible himar1C9 transposase. The backbone encodes for chloramphenicol resistance on a medium copy p15A backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelper_AraC/pBad-Tnpase (camR) was a gift from James Collins (Addgene plasmid # 213909 ; http://n2t.net/addgene:213909 ; RRID:Addgene_213909)

  • For your References section:

    A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks. English MA, Alcantar MA, Collins JJ. Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 10.15252/msb.202211398. Epub 2023 Mar 27. 10.15252/msb.202211398 PubMed 36970845
Related items: