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pDonor_RB-TnV2_pJEx
(Plasmid #213908)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213908 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonor_RB-TnV2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a mariner transposon with a kanamycin resistance gene and an outward-facing crystal violet-inducible promoter on an R6k gamma suicide backbone.. The backbone encodes for ampicillin resistance and can be propagated in pir+ strains (e.g., One Shot PIR1 competent cells). Kanamycin is used to select pir- cells that have undergone a transposition event. N20 Barcodes can be added via the FseI-SbfI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor_RB-TnV2_pJEx was a gift from James Collins (Addgene plasmid # 213908 ; http://n2t.net/addgene:213908 ; RRID:Addgene_213908)

  • For your References section:

    A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks. English MA, Alcantar MA, Collins JJ. Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 10.15252/msb.202211398. Epub 2023 Mar 27. 10.15252/msb.202211398 PubMed 36970845
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