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hsKIF5B(1-560)-HaloTag-FRB
(Plasmid #213899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Chicken beta-actin
  • Backbone size w/o insert (bp) 6062
  • Total vector size (bp) 8924
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsKIF5B(aa1-560)-HaloTag-FRB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2862
  • GenBank ID
    3799
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site SnaBI/XbaI (unknown if destroyed)
  • 5′ sequencing primer ggcttctggcgtgtgaccggcgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid ID: pKJ060/pWS095.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hsKIF5B(1-560)-HaloTag-FRB was a gift from Lukas Kapitein (Addgene plasmid # 213899 ; http://n2t.net/addgene:213899 ; RRID:Addgene_213899)
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