pGWB605-GBSSI
(Plasmid
#213867)
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PurposeBinary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB605
- Total vector size (bp) 14008
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Vector typeBacterial Expression, Plant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGRANULE-BOUND STARCH SYNTHASE I (GBSSI)
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2922
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Entrez GeneGBSS1 (a.k.a. AT1G32900, F9L11.8, F9L11_8, granule bound starch synthase 1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB605-GBSSI was a gift from Yutaka Kodama (Addgene plasmid # 213867 ; http://n2t.net/addgene:213867 ; RRID:Addgene_213867) -
For your References section:
Fluorescein staining of chloroplast starch granules in living plants. Ichikawa S, Sakata M, Oba T, Kodama Y. Plant Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528. 10.1093/plphys/kiad528 PubMed 37792703