pGWB405-TIC21-sGFP
(Plasmid
#213865)
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PurposeBinary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGWB405
- Total vector size (bp) 12277
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Vector typeBacterial Expression, Plant Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namechloroplast inner envelope membrane transit peptide
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1497
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Entrez GeneTIC21 (a.k.a. AT2G15290, ATTIC21, AtTic21, CHLOROPLAST IMPORT APPARATUS 5, CIA5, F27O10.6, F27O10_6, PERMEASE IN CHLOROPLASTS 1, PIC1, TRANSLOCON AT INNER MEMBRANE OF CHLOROPLASTS 21, translocon at inner membrane of chloroplasts 21)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB405-TIC21-sGFP was a gift from Yutaka Kodama (Addgene plasmid # 213865 ; http://n2t.net/addgene:213865 ; RRID:Addgene_213865) -
For your References section:
Live-cell imaging of the chloroplast outer envelope membrane using fluorescent dyes. Ichikawa S, Ishikawa K, Miyakawa H, Kodama Y. Plant Direct. 2022 Nov 15;6(11):e462. doi: 10.1002/pld3.462. eCollection 2022 Nov. 10.1002/pld3.462 PubMed 36398034
