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Addgene

pGWB405-TIC21-sGFP
(Plasmid #213865)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGWB405
  • Total vector size (bp) 12277
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    chloroplast inner envelope membrane transit peptide
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1497
  • Entrez Gene
    TIC21 (a.k.a. AT2G15290, ATTIC21, AtTic21, CHLOROPLAST IMPORT APPARATUS 5, CIA5, F27O10.6, F27O10_6, PERMEASE IN CHLOROPLASTS 1, PIC1, TRANSLOCON AT INNER MEMBRANE OF CHLOROPLASTS 21, translocon at inner membrane of chloroplasts 21)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWB405-TIC21-sGFP was a gift from Yutaka Kodama (Addgene plasmid # 213865 ; http://n2t.net/addgene:213865 ; RRID:Addgene_213865)
  • For your References section:

    Live-cell imaging of the chloroplast outer envelope membrane using fluorescent dyes. Ichikawa S, Ishikawa K, Miyakawa H, Kodama Y. Plant Direct. 2022 Nov 15;6(11):e462. doi: 10.1002/pld3.462. eCollection 2022 Nov. 10.1002/pld3.462 PubMed 36398034