pGWB508-cTP-sfGFP
(Plasmid
#213863)
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PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB508
- Total vector size (bp) 11177
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Vector typeBacterial Expression, Plant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePlastid transit peptide fused sfGFP
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Insert Size (bp)870
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB508-cTP-sfGFP was a gift from Yutaka Kodama (Addgene plasmid # 213863 ; http://n2t.net/addgene:213863 ; RRID:Addgene_213863) -
For your References section:
Using the organelle glue technique to engineer the plant cell metabolome. Ishikawa K, Kobayashi M, Kusano M, Numata K, Kodama Y. Plant Cell Rep. 2023 Mar;42(3):599-607. doi: 10.1007/s00299-023-02982-2. Epub 2023 Jan 27. 10.1007/s00299-023-02982-2 PubMed 36705704