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Addgene

pStorage_Ptet-2_ZF2
(Plasmid #213845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    self-made
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-2 (ZF 42-10).

To insert barcoded genes:
i) digest this plasmid with BbsI and MfeI
ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites
iii) ligate the two digestion products.

Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pStorage_Ptet-2_ZF2 was a gift from James Collins (Addgene plasmid # 213845 ; http://n2t.net/addgene:213845 ; RRID:Addgene_213845)

  • For your References section:

    A high-throughput synthetic biology approach for studying combinatorial chromatin-based transcriptional regulation. Alcantar MA, English MA, Valeri JA, Collins JJ. Molecular Cell, June 2024 10.1016/j.molcel.2024.05.025
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