pStorage_Ptet-2_ZF2
(Plasmid
#213845)
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Purpose(Empty Backbone) CombiGEM storage plasmid for ZF-2 CR fusions.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneself-made
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-2 (ZF 42-10).
To insert barcoded genes:
i) digest this plasmid with BbsI and MfeI
ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites
iii) ligate the two digestion products.
Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pStorage_Ptet-2_ZF2 was a gift from James Collins (Addgene plasmid # 213845 ; http://n2t.net/addgene:213845 ; RRID:Addgene_213845) -
For your References section:
A high-throughput synthetic biology approach for studying combinatorial chromatin-based transcriptional regulation. Alcantar MA, English MA, Valeri JA, Collins JJ. Molecular Cell, June 2024 10.1016/j.molcel.2024.05.025
