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Addgene

pPtet-2-VP16
(Plasmid #213842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    self-made
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VP16
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains one aTc-inducible promoter variants driving the expression of VP-16 fused to ZF-2 (ZF-42-10).

**This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.**

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPtet-2-VP16 was a gift from James Collins (Addgene plasmid # 213842 ; http://n2t.net/addgene:213842 ; RRID:Addgene_213842)

  • For your References section:

    A high-throughput synthetic biology approach for studying combinatorial chromatin-based transcriptional regulation. Alcantar MA, English MA, Valeri JA, Collins JJ. Molecular Cell, June 2024 10.1016/j.molcel.2024.05.025
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