pReporter_mNeonGreen
(Plasmid
#213838)
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PurposeFacilitates genomic integration of the control reporter without zinc finger binding sites.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEU
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains a minimal CYC1 promoter driving expression of mNeonGreen. No ZF binding sites are present.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pReporter_mNeonGreen was a gift from James Collins (Addgene plasmid # 213838 ; http://n2t.net/addgene:213838 ; RRID:Addgene_213838) -
For your References section:
A high-throughput synthetic biology approach for studying combinatorial chromatin-based transcriptional regulation. Alcantar MA, English MA, Valeri JA, Collins JJ. Molecular Cell, June 2024 10.1016/j.molcel.2024.05.025
