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Addgene

pReporter_ZF1_ZF2_mNeonGreen
(Plasmid #213836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEU
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) and ZF-2 (ZF 42-10) are upstream of the promoter.

Cut with NotI prior to transformation into yeast to facilitate genomic insertion.

**This plasmid is prone to recombination due to ZF binding site repeats.**

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pReporter_ZF1_ZF2_mNeonGreen was a gift from James Collins (Addgene plasmid # 213836 ; http://n2t.net/addgene:213836 ; RRID:Addgene_213836)

  • For your References section:

    A high-throughput synthetic biology approach for studying combinatorial chromatin-based transcriptional regulation. Alcantar MA, English MA, Valeri JA, Collins JJ. Molecular Cell, June 2024 10.1016/j.molcel.2024.05.025
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