pAAV_BiCHATe27_GCaMP6f
(Plasmid
#213833)
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PurposeAAV construct expressing GCaMP6f driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_GCaMP6f
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-S5E2-dTom-nlsdTom
- Total vector size (bp) 6356
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP6f
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Insert Size (bp)1350
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_BiCHATe27_GCaMP6f was a gift from Gordon Fishell (Addgene plasmid # 213833 ; http://n2t.net/addgene:213833 ; RRID:Addgene_213833) -
For your References section:
An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes. Furlanis E, Dai M, Leyva Garcia B, Vergara J, Pereira A, Pelkey K, Tran T, Gorissen BL, Vlacho A, Hairston A, Huang S, Dwivedi D, Du S, Wills S, McMahon J, Lee AT, Chang EF, Razzaq T, Qazi A, Vargish G, Yuan X, Caccavano A, Hunt S, Chittajallu R, McLean N, Hewit L, Paranzino E, Rice H, Cummins AC, Plotnikova A, Mohanty A, Tangen AC, Shin J, Azadi R, Eldridge MAG, Alvarez VA, Averbeck BB, Alyahyay M, Reyes Vallejo T, Soheib M, Vattino LG, MacGregor CP, Banks E, Olah V, Naskar S, Hill S, Liebergall S, Badiani R, Hyde L, Xu Q, Allaway KC, Goldberg EM, Nowakowski TJ, Lee S, Takesian AE, Ibrahim LA, Iqbal A, McBain CJ, Dimidschstein J, Fishell G, Wang Y. bioRxiv 2024.07.17.603924 10.1101/2024.07.17.603924