pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast
(Plasmid #213826)

• Purpose: Expresses human PRDM6 with a C-terminal V5 epitope tag.
• Depositing Lab: Bjoern Schwer
• Publication: Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389. doi: 10.1101/2023.08.29.555389. Preprint.

Backbone

• Vector backbone: pCDH-Lenti-CAGGS-MCS-EF1-Luc2-Blast
• Backbone manufacturer: n/a
• Backbone size w/o insert (bp): 10307
• Total vector size (bp): 12143
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRDM6
• Species: H. sapiens (human)
• Insert Size (bp): 1836
• GenBank ID: NM_001136239.4
• Entrez Gene: PRDM6 (a.k.a. KMT8C, PDA3, PRISM)
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry