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Addgene

POC1476
(Plasmid #213802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5421
  • Total vector size (bp) 6705
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BsaI-associated switch methylase M.Sen0738I
  • Alt name
    M.Sen0738I subunit M
  • Species
    S. enterica subsp. enterica serovar Thompson CFSAN000738
  • Insert Size (bp)
    1470
  • GenBank ID
    CP019196
  • Promoter T7
  • Tag / Fusion Protein
    • His6-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CTAGCCATATGTCAATCTCATCTGCTATTAAAAGC
  • 3′ sequencing primer CTAGCTCGAGGTTCGCCTCTTTACCACTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POC1476 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 213802 ; http://n2t.net/addgene:213802 ; RRID:Addgene_213802)

  • For your References section:

    DNA methylases for site-selective inhibition of type IIS restriction enzyme activity. Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA. Appl Microbiol Biotechnol. 2024 Jan 25;108(1):174. doi: 10.1007/s00253-024-13015-7. 10.1007/s00253-024-13015-7 PubMed 38270650
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