pGR596
(Plasmid #213787)

• Purpose: Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
• Depositing Lab: Chang Liu
• Publication: Continuous evolution of user-defined genes at 1-million-times the genomic mutation rate (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Bacterial Expression, Yeast Expression, Synthetic Biology
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BadBoy2 TP-DNAP1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3000
• Mutation: See manuscript

Resource Information

• Supplemental Documents:
  • GR596 - pCAN1int.ISceI.expr-EcoRI.site-BadBoy3(SAC6p)-NatMX.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry