p103_gRNA_Sp_mClover
(Plasmid
#213779)
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PurposeCloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213779 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiviral
- Total vector size (bp) 8655
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Vector typeLentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSp gRNA
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Insert Size (bp)-3
- Promoter mU6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Esp3I (unknown if destroyed)
- 3′ cloning site Esp3I (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p103_gRNA_Sp_mClover was a gift from Howard Chang (Addgene plasmid # 213779 ; http://n2t.net/addgene:213779 ; RRID:Addgene_213779) -
For your References section:
Bidirectional epigenetic editing reveals hierarchies in gene regulation. Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY. Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3. 10.1038/s41587-024-02213-3 PubMed 38760566
Map uploaded by the depositor.
