p101_CRISPRai_PiggyBac
(Plasmid
#213777)
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PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac
- Total vector size (bp) 17168
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Vector typeCRISPR
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameVPR-dSaCas9
- Promoter Tet_On_TRE
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namedSpCas9-KRAB-BFP
- Promoter Tet_On_TRE
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert namezeocin
- Promoter EF1a (EF-1-alpha intron a)
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameTet-On transactivator
- Promoter EF1a (EF-1-alpha intron a)
Cloning Information for Gene/Insert 4
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p101_CRISPRai_PiggyBac was a gift from Howard Chang (Addgene plasmid # 213777 ; http://n2t.net/addgene:213777 ; RRID:Addgene_213777) -
For your References section:
Bidirectional epigenetic editing reveals hierarchies in gene regulation. Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY. Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3. 10.1038/s41587-024-02213-3 PubMed 38760566
Map uploaded by the depositor.
