pLV-HKGT3_mCherry2-NLS-PEST_Puro
(Plasmid
#213771)
-
PurposeFluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 213771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneLentiviral Gene Expression Vector
-
Backbone manufacturerVectorBuilder Inc.
- Backbone size w/o insert (bp) 8249
-
Vector typeLentiviral, Synthetic Biology
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHKGT3_mCherry2-NLS-PEST
-
SpeciesH. sapiens (human)
-
Insert Size (bp)462
Cloning Information
- Cloning method Unknown
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV-HKGT3_mCherry2-NLS-PEST_Puro was a gift from Gaetano Gargiulo (Addgene plasmid # 213771 ; http://n2t.net/addgene:213771 ; RRID:Addgene_213771) -
For your References section:
Logical design of synthetic cis-regulatory DNA for genetic tracing of cell identities and state changes. Company C, Schmitt MJ, Dramaretska Y, Serresi M, Kertalli S, Jiang B, Yin JA, Aguzzi A, Barozzi I, Gargiulo G. Nat Commun. 2024 Feb 5;15(1):897. doi: 10.1038/s41467-024-45069-6. 10.1038/s41467-024-45069-6 PubMed 38316783