pPB-PNGT3-d2EGFP_CMV-mCherry
(Plasmid
#213765)
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PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac Gene Expression Vector
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Backbone manufacturerVectorBuilder Inc.
- Backbone size w/o insert (bp) 6836
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Vector typeSynthetic Biology
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Selectable markersmCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePNGT#3-d2EGFP
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SpeciesH. sapiens (human)
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Insert Size (bp)1062
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-PNGT3-d2EGFP_CMV-mCherry was a gift from Gaetano Gargiulo (Addgene plasmid # 213765 ; http://n2t.net/addgene:213765 ; RRID:Addgene_213765) -
For your References section:
Logical design of synthetic cis-regulatory DNA for genetic tracing of cell identities and state changes. Company C, Schmitt MJ, Dramaretska Y, Serresi M, Kertalli S, Jiang B, Yin JA, Aguzzi A, Barozzi I, Gargiulo G. Nat Commun. 2024 Feb 5;15(1):897. doi: 10.1038/s41467-024-45069-6. 10.1038/s41467-024-45069-6 PubMed 38316783