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Addgene

POC1470
(Plasmid #213758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213758 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5421
  • Total vector size (bp) 6396
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Truncated non-switchable methylase M2.BsaI
  • Alt name
    M2.BsaI_2
  • Species
    B. stearothermophilus 6-55
  • Insert Size (bp)
    1146
  • GenBank ID
    NEBC14812
  • Promoter T7
  • Tag / Fusion Protein
    • His6-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer CTAGCCATATGCTTATCATTAAACACGTGCC
  • 3′ sequencing primer CTAGAAGCTTAATGATAGCTAAGGAGGACAGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POC1470 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 213758 ; http://n2t.net/addgene:213758 ; RRID:Addgene_213758)

  • For your References section:

    DNA methylases for site-selective inhibition of type IIS restriction enzyme activity. Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA. Appl Microbiol Biotechnol. 2024 Jan 25;108(1):174. doi: 10.1007/s00253-024-13015-7. 10.1007/s00253-024-13015-7 PubMed 38270650
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