pHIV-Luciferase
(Plasmid #21375)

• Purpose: (Empty Backbone) self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
• Depositing Lab: Bryan Welm
• Publication: Lentivirus-EF1alpha-MCS-IRES-Luciferase (unpublished)

Backbone

• Vector backbone: pSICO
• Backbone size (bp): 8713
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Growth in Stbl3 is preferred
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: 5'-TGGAATTTGCCCTTTTTGAG-3'
• 3′ sequencing primer: 5'-AGGAACTGCTTCCTTCACGA-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pSICO was obtained from Tyler Jacks laboratory.
• Articles Citing this Plasmid:
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.

NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.