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Addgene

rnLbCas12a-PE2
(Plasmid #213746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-PE2
  • Backbone manufacturer
    David Liu
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rnLbCas12a-M-MLV RT∆RNase H
  • Species
    Synthetic; Lachnospiraceae bacterium
  • Mutation
    D156R, H759A, R1138A in LbCas12a
  • Promoter CMV
  • Tags / Fusion Proteins
    • BPNLS (N terminal on insert)
    • BPNLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rnLbCas12a-PE2 was a gift from Caixia Gao (Addgene plasmid # 213746 ; http://n2t.net/addgene:213746 ; RRID:Addgene_213746)

  • For your References section:

    Prime editing using CRISPR-Cas12a and circular RNAs in human cells. Liang R, He Z, Zhao KT, Zhu H, Hu J, Liu G, Gao Q, Liu M, Zhang R, Qiu JL, Gao C. Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x. 10.1038/s41587-023-02095-x PubMed 38200119
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