mpeg:lamp2-mCherry-stop; cmlc2:GFP
(Plasmid
#213740)
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PurposeExpresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest
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Vector typeZebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelamp2-mCherry
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SpeciesD. rerio (zebrafish)
- Promoter mpeg1.1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mpeg:lamp2-mCherry-stop; cmlc2:GFP was a gift from William Talbot (Addgene plasmid # 213740 ; http://n2t.net/addgene:213740 ; RRID:Addgene_213740) -
For your References section:
A lysosomal regulatory circuit essential for the development and function of microglia. Iyer H, Shen K, Meireles AM, Talbot WS. Sci Adv. 2022 Sep 2;8(35):eabp8321. doi: 10.1126/sciadv.abp8321. Epub 2022 Aug 31. 10.1126/sciadv.abp8321 PubMed 36044568