pHIV-dTomato
(Plasmid #21374)

• Purpose: (Empty Backbone) self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato
• Depositing Lab: Bryan Welm
• Publication: pHIV-dTomato (unpublished)

Backbone

• Vector backbone: pSICO
• Backbone size (bp): 6900
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Growth in Stbl3 is preferred
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: 5'-TGGAATTTGCCCTTTTTGAG-3'
• 3′ sequencing primer: 5'-AGGAACTGCTTCCTTCACGA-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The red fluorescent protein dTomato was obtained from Roger Tsien's laboratory and was published in: Nature Biotechnology 22, 1567 - 1572 (2004) and pSICO was obtained from Tyler Jacks laboratory.
• Articles Citing this Plasmid:
  • 37 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The HIV-dTomato plasmid encodes a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with dTomato. This construct was made in Zena Werb's laboratory and has not been published. Note: This plasmid contains dTomato and not the tandem conjugate TdTomato.