pHIV-EGFP
(Plasmid #21373)

• Purpose: (Empty Backbone) self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP
• Depositing Labs: Bryan Welm, Zena Werb
• Publication: Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102.

Backbone

• Vector backbone: pSICO
• Backbone size (bp): 6900
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Growth in Stbl3 is preferred
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: 5'-TGGAATTTGCCCTTTTTGAG-3'
• 3′ sequencing primer: 5'-AGGAACTGCTTCCTTCACGA-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EGFP was from Clontech and pSICO was from Tyler Jacks.
• Articles Citing this Plasmid:
  • 40 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HIV-EGFP is a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with EGFP. This construct was described in the publication: Cell Stem Cell. 2008 Jan 10. 2(1):90-102.

Information for Cloning Grade DNA (Catalog # 21373-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here